Composition. Such devices should provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer. [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec12035 [Full Text], Cold Spring Harb Protoc; 2017; doi:10.1101/pdb.rec092981 Sodium orthovanadate preparation. [Full Text], Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.rec089730 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec079632 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8505 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11553 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec510 All procedures must be carried out under the fume hood. pH. [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11168 1-Step Transfer Buffer is compatible with Power Blotter and other protein semi-dry transfer devices, when they are paired with a suitable high-current power supply. [Full Text], Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.rec077487 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec512 [Full Text], Triton X-100 Solution for Purification of VDAC1 from Mitochondria, Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec076166 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec105734 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8807 Tris Glycine Transfer Buffer with 20% methanol. [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec067264 Transfer … Dissolve the following reagents in 400 mL ultrapure water. [Full Text], Cold Spring Harb Protoc; 2013; doi:10.1101/pdb.rec073627 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11983 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec105064 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec416 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12138 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec077420 Copyright © 2020 by Cold Spring Harbor Laboratory Press. [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11948 [Full Text], Transcription Buffer for RNA Polymerase (5x), Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.rec072504 Western Blotting: Remove the membrane from the transfer apparatus and place in 20 ml of 5% non-fat dry milk in TBST for one hour, with gentle shaking. 2) Add methanol and mix. [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12158 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11118 Dissolve the following reagents in 100 mL ultrapure water. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8429 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11570 [Full Text], Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.rec089276

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